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  • 首頁 >> 產(chǎn)品展示 >> 生化試劑 >> 海外試劑代購(gòu) >> thermo二抗A21203|Invitrogen抗體*

    thermo二抗A21203|Invitrogen抗體*

    簡(jiǎn)要描述:thermo二抗A21203|Invitrogen抗體*
    Alexa Fluor染料是目前較值得信賴的熒光染料之一。 Invitrogen™Alexa Fluor 594染料是一種明亮的紅色熒光染料,具有理想的激發(fā),適用于594 nm激光線。

    • 產(chǎn)品型號(hào):
    • 廠商性質(zhì):經(jīng)銷商
    • 更新時(shí)間:2019-04-19
    • 訪  問  量:1948
    詳細(xì)資料

    thermo二抗A21203|Invitrogen抗體*

     

    Alexa Fluor染料是目前值得信賴的熒光染料之一。 Invitrogen™Alexa Fluor 594染料是一種明亮的紅色熒光染料,具有理想的激發(fā),適用于594 nm激光線。為了在成像和流式細(xì)胞儀中產(chǎn)生穩(wěn)定的信號(hào),Alexa Fluor 594染料在很寬的摩爾范圍內(nèi)對(duì)pH不敏感。具有高熒光量子產(chǎn)率和高光穩(wěn)定性的探針允許以高靈敏度檢測(cè)低豐度生物結(jié)構(gòu)。 Alexa Fluor 594染料分子可以高摩爾比附著在蛋白質(zhì)上而不會(huì)發(fā)生明顯的自猝滅,從而實(shí)現(xiàn)更明亮的結(jié)合物和更靈敏的檢測(cè)。每種綴合物的標(biāo)記程度通常為每個(gè)IgG分子2-8個(gè)熒光團(tuán)分子;每個(gè)產(chǎn)品批次的分析證書上都標(biāo)明了確切的標(biāo)簽程度。

    這些驢抗小鼠IgG(H + L)全二級(jí)抗體已經(jīng)過親和純化,并顯示出與牛,雞,山羊,豚鼠,倉鼠,馬,人,兔,大鼠和綿羊血清蛋白的小交叉反應(yīng)性。交叉吸附或預(yù)吸附是提高抗體特異性的純化步驟,導(dǎo)致更高的靈敏度和更少的背景染色。使第二抗體溶液通過含有來自潛在交叉反應(yīng)性物質(zhì)的固定化血清蛋白的柱基質(zhì)。在柱中僅捕獲非特異性結(jié)合的二抗,并且高度特異性的第二抗體流過。這種額外步驟的益處在多重/多色染色實(shí)驗(yàn)(例如,流式細(xì)胞術(shù))中是顯而易見的,其中存在與其他一抗的潛在交叉反應(yīng)性或在可能存在內(nèi)源免疫球蛋白的組織/細(xì)胞熒光染色實(shí)驗(yàn)中。

     

    thermo二抗A21203|Invitrogen抗體*

     

     

    Product Details

    Tested Applications

    Dilution

    Immunocytochemistry (ICC)

    4 µg/mL

    Immunofluorescence (IF)

    4 µg/mL

    Immunohistochemistry (IHC)

    1-10 µg/mL

    Published Applications

    Immunohistochemistry (IHC)

    See 5 publications below

    Immunocytochemistry (ICC)

    See 6 publications below

    Immunohistochemistry (Frozen) (IHC (F))

    See 3 publications below

    Miscellaneous PubMed (MISC)

    See 14 publications below

    Product Specifications

    Species Reactivity

    Mouse

    Host / Isotype

    Donkey / IgG

    Class

    Polyclonal

    Type

    Secondary Antibody

    Immunogen

    Gamma Immunoglobins Heavy and Light chains

    Conjugate

    Alexa Fluor® 594

    Excitation/Emission Profile

    View spectra 

    Form

    Liquid

    Concentration

    2 mg/mL

    Purification

    purified

    Storage buffer

    PBS, pH 7.5

    Contains

    5mM sodium azide

    Storage conditions

    4° C, store in dark

    RRID

    AB_2535789

    Target

    IgG

    Antibody Form

    Whole Antibody

    Target Information

     

    We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.

    Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.

    Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.

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